Cell Counting & Seeding Calculator
How to Use:
1. Cell Counting: Input the hemocytometer counts and dilution factor (e.g., Trypan Blue) to find your current cell concentration.
2. Seeding Setup: Enter the target density and volume for your culture plate (e.g., 6-well, 10cm dish). Scientific notation (e.g.,
3. Click Calculate to generate the Master Mix volume required for uniform seeding.
1. Cell Counting: Input the hemocytometer counts and dilution factor (e.g., Trypan Blue) to find your current cell concentration.
2. Seeding Setup: Enter the target density and volume for your culture plate (e.g., 6-well, 10cm dish). Scientific notation (e.g.,
3e5 for 300,000) is supported.3. Click Calculate to generate the Master Mix volume required for uniform seeding.
1. Hemocytometer Cell Count
2. Cell Seeding Parameters
Calculation Result & Master Mix Preparation
Current Cell Concentration
0.00 × 10^0 cells/mL
| Master Mix Component (for wells*) | Volume (mL) |
|---|---|
| Original Cell Suspension | 0.00 mL |
| Fresh Culture Media | 0.00 mL |
| Total Master Mix Volume | 0.00 mL |
* Calculated with a % excess margin to compensate for pipetting loss. Mix the master mix thoroughly and aliquot mL into each well.