SDS-PAGE Gel Recipe Generator
How to Use:
1. Select the desired Resolving Gel Percentage based on your target protein size.
2. Enter the number of gels you want to cast. (A 10% pipetting margin is automatically included).
3. Click Calculate to generate the exact volumes for both Resolving and Stacking gels.
Resolving Gel (10%)
Component Volume (Master Mix)
ddH₂O 0.00 mL
30% Acrylamide/Bis 0.00 mL
1.5 M Tris-HCl (pH 8.8) 0.00 mL
10% SDS 0 µL
10% APS (Add right before casting) 0 µL
TEMED (Add right before casting) 0 µL
Total Volume 0.00 mL
Stacking Gel (5%)
Component Volume (Master Mix)
ddH₂O 0.00 mL
30% Acrylamide/Bis 0.00 mL
0.5 M Tris-HCl (pH 6.8) 0.00 mL
10% SDS 0 µL
10% APS (Add right before casting) 0 µL
TEMED (Add right before casting) 0 µL
Total Volume 0.00 mL
* Note: Recipes are calculated using a standard Laemmli buffer system. A 10% margin is added to the total volume to prevent shortage during casting. Always add APS and TEMED last, as polymerization will begin immediately.