Oligo & Primer Resuspension Calculator
How to Use:
Calculate the exact volume of TE buffer or Nuclease-free water required to reconstitute lyophilized primers, ASOs, or siRNA to your target stock concentration.
* Use Method 1 for standard PCR primers (amount provided in nmol).
* Use Method 2 for custom ASOs or modified oligos (amount provided in µg or mg with Molecular Weight).
Calculate the exact volume of TE buffer or Nuclease-free water required to reconstitute lyophilized primers, ASOs, or siRNA to your target stock concentration.
* Use Method 1 for standard PCR primers (amount provided in nmol).
* Use Method 2 for custom ASOs or modified oligos (amount provided in µg or mg with Molecular Weight).
1. By Moles (Standard PCR Primers)
Resuspension Volume
Add Solvent Volume:
0.00 µL
💡 Rule of Thumb: To make a standard 100 µM stock solution, simply multiply the nmol amount by 10 to get the volume in µL. (e.g., 34.5 nmol × 10 = 345 µL).
2. By Mass & Molecular Weight (ASOs, Custom Oligos)
Resuspension Volume
Add Solvent Volume:
0.00 µL
* We recommend resuspending modified oligos (like ASOs or 2'-OMe modified RNA) in TE buffer (pH 8.0) rather than water to ensure long-term stability and prevent degradation.