[LabTool] Protein Concentration Calculation

BCA Protein Assay Normalization Tool

How to Use:
1. Enter your assay parameters (Dilution factor, desired Final Volume, Loading Buffer stock).
2. Paste concentrations directly from Excel, or type them in manually.
3. Click Calculate Normalization to generate the exact pipetting recipe for equal protein loading across all lanes.

1. Assay & Normalization Settings

Assay Dilution Factor e.g. 5 if 5 μL lysate + 20 μL diluent

Final Normalized Vol (μL) Total volume to prepare per sample

Loading Buffer Stock 4X SDS Loading Buffer 5X SDS Loading Buffer 6X SDS Loading Buffer 2X SDS Loading Buffer To achieve 1X working concentration

2. Sample Concentrations (μg/mL) Accepts plate reader outputs

💡 You can paste a single column of numbers, or two columns with Sample Name and Concentration.

3. Normalization Pipetting Recipe

Sample Name	Raw Conc. (μg/mL)	Corrected Conc. (μg/μL)	Lysate Vol (μL)	Lysis Buffer (μL)	Loading Buffer (μL)	Total Vol (μL)

Target Normalized Concentration

0.00 μg/μL

Determined by the lowest concentration sample

Gel Loading Guide (Target: 30 μg)

0.0 μL

Volume to load for equal protein per well

Loading Buffer per Tube

0.0 μL

Constant across all samples